Aflatoxin total (B group G) immunoaffinity column (AFT-IAC) instruction manual

Total aflatoxins (B Group G Group) immunoaffinity column (AFT-IAC)

1. Product introduction

Aflatoxin (AFT) is a carcinogenic and mutagenic substance that poses a great threat to human health. Therefore, countries have strict limits on the content of aflatoxin (AFT) in food. This product cross-links specific monoclonal antibodies against aflatoxin B1, B2, G1, G2 on agarose gel microspheres. Based on the specific binding of antigen-antibody, the sample is purified and aflatoxin B1 is prepared. B2, G1, and G2 are specifically bound to the immunoaffinity column, and other substances are eluted, and then the contents of aflatoxins B1, B2, G1, and G2 in the sample are determined by elution, elution, collection, and detection.

The efficiency, recovery rate and maximum load of this product are in line with national standards:

GB 5009.22-2016 (Determination of aflatoxin B and G in foods for food safety national standards)

GB/T 30955-2014 (Determination of aflatoxins B1, B2, G1, G2 in feed - Immunoaffinity column purification high performance liquid chromatography) GB/T 18979-2003 (Aflatoxin determination in food - Immunoaffinity Chromatography purification high performance liquid chromatography and fluorescence spectrophotometry)

2. Use

Applicable to food (such as: corn, peanuts and their products, rice, wheat, vegetable oils, soy sauce, vinegar, alcohol, dairy products, etc.), feed, herbs, tea, aflatoxin B1, B2, G1, G2 content Determination; purification, extraction, concentration, etc. of aflatoxins B1, B2, G1, and G2 in the sample.

3. Product performance

4. Operation process

1 Sample treatment: The samples were treated according to the respective methods in GB 5009.22-2016 (Determination of aflatoxin B and G in the food safety national standard food).

2 Purification: Take GB 5009.22-2016 (measurement of aflatoxin B and G in food safety national food) as an example (1) Column pretreatment: remove the total aflatoxin immunoaffinity column and restore to room temperature (22-25 °C), remove the upper end plug, open the lower end plug, and drain the preservation solution in the column to 3-5 mm above the interface on the gel to stop the flow (or connect the immunochromatographic column directly to the 20 ml syringe) under).

(2) Adding sample: Add the processed sample liquid through the column one after another, open the lower end plug, adjust the flow rate to support 1-2 drops/second, and discard the effluent. Note: rice, corn, wheat, peanuts and their products, vegetable oil: add 15ml of soy sauce, vinegar: add 10ml of processed sample (3) rice, corn, wheat, peanuts and their products, plant oil samples rinse : After the liquid is completely drained, use 10ml

Distilled water, rinse twice, discard the effluent. Soy sauce and vinegar samples were rinsed: After the liquid was completely drained, wash it with 10 mL of 0.1% Tween-20 in PBS, discard the effluent; rinse with 10 ml of distilled water twice, discard the effluent.

(4) Blow dry: Use vacuum equipment (such as suction balls, syringes, etc.), press in the air, and dry the water in the column.

(5) Elution: Add 1 ml of methanol (chromatographic grade), adjust the flow rate to 2-3 seconds per drop, slowly elute, and collect all the eluate (ie, the purified sample).

3 detection

The purified sample can be directly detected by ELISA, fluorescence and high performance liquid phase.