Disposable exfoliated cell stick dispenser manufacturers wholesale product use details
The exfoliated cell stick remover directly adheres to the contact-type exfoliated cells on the surface of the permeable and non-permeable objects by means of tape, and can be widely used for site extraction and extraction and preservation of trace biological evidence in the forensic DNA laboratory.
The tape is fixed on the seat holder for easy operation. Before use, the rubber surface is covered with a protective film, which is stored in the plexiglass closed container before and after use to avoid contamination.
1. Open the sampling box cover and remove the protective layer with a clean tweezers
2, holding a white plastic base, the glue surface is aligned with the sample extraction part, and repeatedly sticking 10-15 times
3. After sampling, cover the cover in time
4. Open the lid in the DNA extraction area.
Exfoliated cell sticker manual steps
1. Place an appropriate amount of the test sample into a 1.5 ml sample tube.
2. Add 190 μl of buffer G2.
3. Add 10 μl of proteinase K and vortex for 10 seconds.
Fine spot, cartilage, etc. need to add DTT, you can add PK appropriately, the total volume of 2, 3 steps should not exceed 300μl.
4. Incubate at 56 ° C for 1 hour. The incubation time of fine spots and cartilage is appropriately extended. (In the course of the incubation, remove the oscillation several times.)
5. Centrifuge at 13,000 rpm for 5 minutes and transfer 200 μl of supernatant to a 1.5 ml sample tube.
6. MTL added in the sample tube, MTL: sample solution = 3:1, shake the magnetic beads and then add 30 μl of magnetic beads, mix the solution thoroughly, and adsorb for 15 minutes (shake once every 2 minutes).
7. After mixing, place the sample tube on the magnetic stand, make the magnetic frame and sample reverse 2 or 3 times, aspirate the solution, add 500 μl of MW1 solution, shake the sample solution, and aspirate the solution on the magnetic stand.
8. Add 500 μl of 80% ethanol solution, shake the sample solution, place on a magnetic stand, and invert the magnetic stand and sample 2 or 3 times to aspirate the solution.
9. Repeat step 8 twice to achieve 3 washes. After the last wash, remove all wash solution.
The exfoliated cell stick remover directly adheres to the contact-type exfoliated cells on the surface of the permeable and non-permeable objects by means of tape, and can be widely used for site extraction and extraction and preservation of trace biological evidence in the forensic DNA laboratory.
The tape is fixed on the seat holder for easy operation. Before use, the rubber surface is covered with a protective film, which is stored in the plexiglass closed container before and after use to avoid contamination.
1. Open the sampling box cover and remove the protective layer with a clean tweezers
2, holding a white plastic base, the glue surface is aligned with the sample extraction part, and repeatedly sticking 10-15 times
3. After sampling, cover the cover in time
4. Open the lid in the DNA extraction area.
Exfoliated cell sticker manual steps
1. Place an appropriate amount of the test sample into a 1.5 ml sample tube.
2. Add 190 μl of buffer G2.
3. Add 10 μl of proteinase K and vortex for 10 seconds.
Fine spot, cartilage, etc. need to add DTT, you can add PK appropriately, the total volume of 2, 3 steps should not exceed 300μl.
4. Incubate at 56 ° C for 1 hour. The incubation time of fine spots and cartilage is appropriately extended. (In the course of the incubation, remove the oscillation several times.)
5. Centrifuge at 13,000 rpm for 5 minutes and transfer 200 μl of supernatant to a 1.5 ml sample tube.
6. MTL added in the sample tube, MTL: sample solution = 3:1, shake the magnetic beads and then add 30 μl of magnetic beads, mix the solution thoroughly, and adsorb for 15 minutes (shake once every 2 minutes).
7. After mixing, place the sample tube on the magnetic stand, make the magnetic frame and sample reverse 2 or 3 times, aspirate the solution, add 500 μl of MW1 solution, shake the sample solution, and aspirate the solution on the magnetic stand.
8. Add 500 μl of 80% ethanol solution, shake the sample solution, place on a magnetic stand, and invert the magnetic stand and sample 2 or 3 times to aspirate the solution.
9. Repeat step 8 twice to achieve 3 washes. After the last wash, remove all wash solution.
Sd Card Storage Ptz Camera,1080P Driving Recorder,Industry Outdoor Camera,Vision Wireless Camera
Shenzhen Zuomi Technology Co., Ltd. , https://www.nbbkvis.com