First, sample preparation
1. Prepare the cultured cells (5 X 106 cells) in a 25cm2 cell culture flask or 60mm cell culture dish.
2. Carefully add xx ml of pre-cooled SBJ cleaning solution (Reagent A) to cover the growth surface
3. Carefully remove the cleaning solution
4. Use a cell scraping rod to gently scrape cells
5. Add xx ml of SBJ cleaning solution (Reagent A) and mix the cells
6. Transfer to a pre-cooled 15 ml conical centrifuge tube (note: suspension cells start from this step)
7. Centrifuge in a 4°C benchtop centrifuge for 5 minutes at 300g
8. Carefully remove the supernatant
9. Add xx microliters of SBJ Lysis Buffer (Reagent B) and mix thoroughly
10. Transfer to pre-cooled 1.5 ml centrifuge tube
11. Strong vortex oscillation for 15 seconds
12. Incubate in an ice bath for 30 minutes
13. Centrifuge in a 4°C micro tabletop centrifuge for 5 minutes at 16000g (or 13000RPM)
14. Carefully remove 500 μl of supernatant into a new pre-cooled 1.5 mL tube
15. 2 μl of protein was taken for quantitative determination of protein
16. Immediately put in -70 ° C for storage or placed in an ice trough to continue the subsequent operations. 2. Cellular activity determination of phosphorous colorimetric quantitative test kit
1. Prepare the sample to be tested (eg cell lysis extract, etc.) and place in an ice bath (note: the sample must be clarified)
2. Set the spectrophotometer (temperature is 30 ° C): the wavelength is 660nm, and set to zero
3. Prepare 5 1.5 ml centrifuge tubes labeled 1 to 5
4. Add SBJ negative solution (Reagent D) and SBJ standard solution (Reagent H) to each centrifuge tube according to the following table, and mix well.
5. Place tubes 1 to 5 in the ice tank for use in standby to avoid light; the standard tube concentration is shown in the table below:
Tube No. SBJ Negative Solution (Reagent D) SBJ Standard Solution (Reagent H) Determination System Standard Phosphorus Concentration
1 0 xx microliters 20 micromoles/liter
2 xx microliters xx microliters 15 micromoles/liter
3 xx microliters xx microliters 10 micromoles/liter
4 xx microliters xx microliters 5 micromoles/liter
5 xx microliters 0 0
Many people always love to use mocking tone to say other people: "You have neuropathy." in real life, It is essentially different with medical psychosis. First of all, it is clear that mental illness is a mental incapacity, behavioral abnormalities as the main features of the disease, regardless of any approach, mental patients have no civil capacity, but also can not be founding, more and more studies show that psychosis is from the pre-born which is a neurological disease caused by a genetic factor (gene) that has changed. The treatment of psychosis mainly take drug, behavioral therapy, work therapy, recreational therapy, psychological treatment and other aspects of grooming to eliminate or reduce the various obstacles to the patient. In addition, diet therapy is also a very good choice.In recent years, anti-psychotropic drugs continue to make a breakthrough, a variety of new drugs emerge in an endless stream, to bring more treatment of mental illness.
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