Scientists successfully achieve single-sperm high-throughput capture using microfluidic chips

Recently, biologists used Fluidigm's C1TM Single-Cell Auto Prep System to successfully capture and isolate dozens of individual sperm cells for downstream single-cell studies on a C1 microfluidic chip.

Figure 1 Sperm microscopy imaging captured by C1 microfluidic chip

Using the pioneering microfluidic technology of the C1TM Single-Cell Auto Prep System to study individual sperm cells will greatly enhance the experimental efficiency of the researchers. This technology seamlessly integrates capture, lysis, nucleic acid sample preparation, etc., enabling researchers to obtain up to 96 single-cell nucleic acid samples for downstream studies in less than a day, with manual processing time reduced to 1 hour . At the same time, since the single-cell sample preparation process is automatically completed by the instrument-controlled microfluidic chip, the difference caused by manual operation is avoided, and the nanoliter (nl)-level reaction system design (Fig. 2) can also help researchers reduce reagents. Consumption.

Figure 2 Schematic diagram of a nanoliter (nl) stage reaction chamber designed independently for each single cell on a C1 microfluidic chip

From 1677, Lewin Hook first observed human living sperm to Stanford University researchers to complete human sperm sequencing, and technological innovations continue to accelerate the process of scientific discovery, hoping that with the help of C1, biologists can be faster. New achievements in the field of sperm research.

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