Determination of content
Take the product powder 0.5g [at the same time another product powder to determine moisture (Appendix IXH first method)], accurately weighed, add ethanol 30ml, soak for 30 minutes, sonicate for 30 minutes, filtered, the residue was washed with the right amount of ethanol 3 Next, the wash solution was incorporated into the filtrate, evaporated to dryness, and the residue was dissolved with absolute ethanol to be transferred to a 5 ml volumetric flask. Absolute ethanol was added to the mark and shaken as a test solution. Another appropriate amount of dehydration andrographolide, andrographolide reference substance, accurately weighed, plus ethanol was made into 1mg each 1ml solution, as the reference solution. According to the thin layer chromatography (Appendix VIB) test, precision to draw the test solution 2 ~ 4μl, the above two kinds of control solution 1μl and 3μl, respectively, in the intersection point of the same carboxymethylcellulose sodium gluconate GF254 thin The plates were developed with chloroform-ethyl acetate-methanol (4:3:0.4) as a developer, removed, dried, and scanned by thin-layer chromatography (Appendix VIB thin-layer scan) to dehydrate andrographolide. Wavelength: λ s = 263 nm, λ R = 370 nm, andrographolide wavelength: λ s = 228 nm, λ R = 370 nm The integrated value of the absorbance of the test sample and the absorbance of the reference substance were measured and calculated.
Calculated on a dry basis, the total amount of dehydroandrographolide (C20H28O4) and andrographolide (C20H30O5) must not be less than 0.80%
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