"Plant tissue culture" technology made a breakthrough in Shandong

Recently, Shandong Golden Seed Crops Co., Ltd. undertook the “Open Plant Tissue Culture” project and passed the technical appraisal of the appraisal committee composed of 9 experts including the Academician of the Chinese Academy of Engineering and Professor Chen Junyu, a doctoral tutor of Beijing Forestry University. "Open plant tissue culture" is a new technique that does not require autoclaving, inoculation with ultra-clean benches, artificial light culture, and completes the differentiation and rooting of tissue culture seedlings in ordinary sunlight greenhouses. At present, the widely used plant tissue culture is carried out in a closed, high-humidity, sterile environment. This tissue culture method has four drawbacks: it is difficult to solve the culture medium contamination of tissue culture seedlings, the cost of tissue culture seedlings is too high, and the scale production technology Immature and tissue culture seed production techniques are cumbersome. After five years of scientific and technological research, Shandong Jinqiu Seed Industry has developed a plant-derived antibacterial medium that does not affect the normal growth of plant tissues, is inexpensive, and has stable performance, and has achieved open tissue culture that is free from high-pressure, ultra-clean benches. The project realized the transfer of plant tissue culture from a sterile laboratory to a bacteria-containing environment, which simplifies the tissue culture procedure, reduces tissue culture costs, improves tissue culture efficiency, and solves the problem of bacterial infections. Major original inventions. Taking cultivation of potato and apple seedlings as an example, the cost per plant is only 0.060 yuan and 0.0541 yuan, which is 62.26% and 66.46% lower than that of traditional tissue culture. The production of millions of plants saves 102,200 kilowatt hours, and the energy saving rate reaches 95%. The average pollution rate of seedlings dropped from 7.6% to 2.5%. The appraisal experts unanimously agreed that the topics chosen for the project are of great significance and that technology is the first of its kind at home and abroad. It is a major breakthrough in the history of plant tissue culture technology. The research success of this project has made the popularization of high-precision technologies widely used in industries such as plant detoxification, protection of germplasm resources, breeding, and extraction of Chinese herbal medicines, which is conducive to ecological environmental governance and genetic transformation.

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