A rare and rare Chinese herbal medicine cultivation techniques, especially the cultivation techniques of fish guts and young leaves. The method is characterized in that it uses the stem segments or apical buds of fish gall bladder and green leaf gallbladder as explants. After routine sterilization, the callus is induced and the differentiation of buds is cultured in a culture medium, and then proliferation culture is used. Basal proliferation culture, and finally with rooting medium for rooting culture. The culture temperature was 20-22°C and the illumination was 14-15 h/day. When the seedling height 3 ~ 5cm when the transplant began, the survival rate of more than 99.6%, growth period is about 1 year. Its ecological, social and economic benefits are considerable.
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