According to the news of the Information Office of the Ministry of Agriculture and Rural Affairs, from the beginning of the first African pig swine outbreak in early August, more than 10 provinces in the country have found outbreaks. The latest release on November 17, Shanghai Jinshan District has detected the African swine fever epidemic: Fifty pigs died of 11 deaths.
Non- African swine fever (ASF) disease is caused by African swine fever virus (ASFV), which infects domestic pigs, warthogs and South African wild boars. The disease is transmitted by direct contact between healthy and diseased animals, or by indirect contact with infected feed, and by biological carriers (soft mites). There are very acute, acute, subacute and chronic forms of onset, of which acute disease has a short incubation period of about 3-7 days, accompanied by high fever (up to 42 ° C), and death within 5-10 days. Due to the virulence of the virus infected by pigs, the mortality rate ranges from 0% to 100%. Any regional or national outbreak has a devastating effect on the local pig breeding industry and related industries. However, it has not been confirmed yet. ASF has the risk of infecting people.
Thermo Fisher's detection program for African swine fever:
From sample to result
1. Easy sample collectionThe GenoTube swab sampling tube , validated by the German FLI (Friedrich-Loeffler-Institute), can be used for sample collection of African swine fever. The sampling tool can realize the sample temperature transportation and storage, and the docking laboratory qPCR, ELISA, colloidal gold detection card detection. Improve biosafety during sample transportation, increase logistics speed while reducing logistics costs.
Figure 1. GenoTube swab sampling tube
2. Fast sample extractionThermo Fisher TM KingFisher Flex TM high-throughput automated nucleic acid extraction system that can perform high-quality extraction of 96 samples in 30 minutes, solving the extraction requirements of customers' massive samples; eliminating the need for centrifugation, liquid transfer, and artificial reduction during nucleic acid extraction Operation time and manual error help users to free up labor.
Figure 2. KingFisher Flex high-throughput automated nucleic acid extraction system
3. High purity nucleic acid purificationThe MagMAX Nucleic Acid Extraction Kit provides high-purity nucleic acids for docking and accurate qPCR detection.
Figure 3. MagMAX Nucleic Acid Extraction Kit
4. Accurate and reliable detection
Figure 4. Applied Biosystems 7500 Real-Time PCR System
VetMAX African swine fever (ASF) qPCR detection reagent was detected for a random combination of 58 ASFV strains from different regions, showing 100% sensitivity and specificity, including between 2007 and 2014 in the Caucasus The Georgian strain (Georgia 2007) that broke out in Russia and its neighboring countries. The first strain isolated from African swine fever reported in China is homologous to the strain. VetMAX African swine fever (ASF) qPCR detection reagent is widely used in the detection of European swine fever outbreaks in Europe, and the test results are reliable.
5. Monitoring system for false negative results In the qPCR assay, ABI's unique internal positive control, Xeno IPC , was added to monitor the entire process from sample preparation to qPCR detection to monitor the appearance of false negative results. It is of great significance for the monitoring of such major diseases. The VetMAX range of kits comes with Xeno IPC.
试剂 Reagents and consumables
*Only for RUO, not for clinical diagnosis
From sample to results, Thermo Fisher offers a total solution for African swine fever testing.
For more product information, please visit the official website of 昊north (Simofei Beijing and Northeast Partner) or follow us on the official WeChat platform
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