[Background introduction]
Recently, Professor Xing Da and Associate Professor Zhang Tao (co-author of communication) of South China Normal University have developed multifunctional functions by combining photothermia (PTT) and photoacoustic therapy (PAT) based on tumor-targeted nano-agents (DBCO-ZnPc-LP). Photothermal therapy. Nanoscale DBCO-ZnPc-LP can be easily prepared by self-assembly of a single lipophilic near-infrared (NIR) dye zinc(II)-phthalocyanine (ZnPc) with lipid poly(ethylene glycol) (LP), and subsequently Further modification of dibenzylcyclohexyl (DBCO) for the introduction of a two-step chemical tumor targeting strategy based on metabolic sugar engineering and click chemistry. The prepared DBCO-ZnPc-LP can not only convert NIR light into heat for effective thermal ablation, but also induce enhanced ultrasonic shock wave to induce local mechanical damage and achieve synergistic anti-tumor effect in vitro and in vivo. In addition, DBCO-ZnPc-LP can be efficiently delivered to tumor cells and solid tumors after intravenous injection through a two-step tumor targeting strategy. Related results are published on ACS nano under the title "In Vivo Imaging-Guided Photothermal/Photoacoustic Synergistic Therapy with Bioorthogonal Metabolic Glycoengineering-Activated Tumor Targeting Nanoparticles".
[Graphic introduction]
Figure 1 DBCO-ZnPc-LP scheme construction
a) Scheme construction of multifunctional nano-scale DBCO-ZnPc-LP
b) The particle size of DBCO-ZnPc-LP was found to be ≈164.18nm
c, d) Optical properties of DBCO-ZnPc-LP were studied by UV-Vis absorption and emission spectroscopy
Figure 2 In vivo photoacoustic and photothermal images
(a) Photoacoustic images of intravenous injection of DBCOZnPc-LP tumors in nude mice at 6, 12 and 24 hours
(b) Thermographic images of 808nm continuous laser (0.1W / cm 2 ) taken at different times
(c) (a) Average PA signal intensity of tumor tissue
(d) Temperature change of the tumor during laser irradiation as a function of irradiation time
Figure 3 PTT / PAT in tumor-bearing mice
(a) Pictures of mice in each group before and after treatment
(b) Measurement of tumor growth after 18 days of treatment
(c) Changes in body weight of mice after various treatments
Figure 4 histological analysis of tumors after various treatments
(a) H&E-stained tumor sections from different treatment groups
(b) Histological staining of hearts, liver, spleen, lungs and kidneys from different groups
ã€summary】
The study can accurately locate solid tumors by integrating targeted strategies, photoacoustic imaging, and synergistic interactions between PTT and PAT, and eradicate them completely in the body. Therefore, this multifunctional photothermal therapy plays an important role in future therapy by enhancing the synergy between PTT and PAT under the guidance of photoacoustic imaging.
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