Application scheme and case of LIBS element analysis technology

1. Life science application programs and cases

The CEITEC/AtomTrace LIBS research team has long been concerned with the application of LIBS technology in the life sciences including biomedical applications.

In 2005, Dr. Jozef Kaiser (Scientific Director of Atomtrace, Professor of Brno University, Head of Laser Spectroscopy Laboratory, Director of the CEITEC Department of Physical Properties and Surface Science) published "Mapping of the metal intake in" in the European Physical Journal. Plants by large-field X-ray microradiography and preliminary feasibility studies in microtomography ” (Eur.Phys. J. D 32, 113-118); 2006, using LIBS femtosecond laser spectroscopy to study and analyze plant samples of iron and manganese The distribution of elements and the first two-dimensional distribution of complete leaf iron elements, and published "Femtosecond laser spectrochemical analysis of plant samples" (Laser Phys.Lett.3, No.1, 21-25, 2006)

From 2007 to 2010, the research team represented by Dr. Kaiser used μCT and femtosecond laser induced breakdown spectroscopy (LIBS) and LA-LCP-MS to study the absorption and accumulation of heavy metals in sunflower and other plant tissues. analysis. The CEITEC/Atomtrace LIBS research team also used LIBS technology to combine three-dimensional chemical imaging with micro-CT (μCT). The above research results have established the leading position of LIBS technology in the laser spectroscopy laboratory represented by Dr. Kaiser and others in the field of global life and environmental science applications.

In 2010, the laser spectroscopy laboratory represented by Kaiser used LIBS technology combined with μCT technology to study and analyze the distribution of spine bone elements including Ca, Al, P, Na, etc. of snake deformity osteitis, and published "Investigation of The osteitis deformans phases in snake vertebrae by double-pulse laser-induced breakdown spectroscopy" (Anal. Bioanal. Chem. 398:1095-1107, 2010)

The laboratory research team also studied the application of LIBS technology in paleontology and archaeology. The following is the analysis of prehistoric animal teeth by laser-induced breakdown spectroscopy and laser ablation inductively coupled (see: Multielemental analysis of prehistoric animal teeth by laser-induced breakdown spectroscopy and laser ablation inductively coupled Applied mass spectrometry. Applied Optics 49 No. 13: 191-199, 2010), this study is equally important for the study of annual ring element distribution and environmental change.

In 2012, Dr. Kaiser and others published a review article "Trace elemental analysis by Laser-induced breakdown spectroscopy---biological applications" based on years of research and peer research (Surface Science Report 67:233-243, 2012) The laboratory also used LIBS technology to conduct extensive research on algae (see “Liquid LIBS Elemental Analysis Application Cases and Protocols” later) and published a review article in 2014 “Algal biomass analysis by laser-based analytical techniques---a Review" (Sensor 14, 2014)

2. Geology and mineral application

The CEITEC/Atomtrace Laser Spectroscopy Laboratory has conducted extensive research on the application of LIBS in geology and mineral resources. In 2014, "Two dimensional elemental mapping by laser-induced breakdown spectroscopy" (Spectroscopy Europe 26 No. 6:6-10, In the 2014 paper, the surface element distribution of arsenic platinum ore, chalcopyrite Pt, Pb, Ni, etc. was constructed in 2D and even combined with μCT.

Uranium is the most promising source of energy for humans. The in situ detection of the presence or absence of uranium is extremely important. The main challenge of uranium detection is its complex spectral emission and high-density spectral curves – even beyond the general spectrometer detection capabilities. The CEITEC Laser Spectroscopy Laboratory used LIBS technology to measure and analyze sandstone uranium deposits through ANN (Artificial Neural Network) information processing technology and PCA (Principal Component Analysis).

3, liquid (aqueous suspension) LIBS element analysis

As early as 2000, Ota Samek and Dr. Jozef Kaiser of the Laser Spectroscopy Laboratory of Brno University of Technology published "Application of laser-induced breakdown spectroscopy to in situ analysis of liquid samples" (Opt.Eng. 39(8) 2248 -2262, 2000). In 2012, the CEITEC Laser Spectroscopy Laboratory Research Team, in collaboration with PSI, published "Application of laser-induced breakdown spectroscopy to the analysis of algal biomass for induxrial biotechnology" in Spectrochimica Acta Part B, which uses LIBS technology and The special liquid sample laser chamber analyzes the accumulation of potassium, magnesium, calcium, sodium and other elements essential for algae and the accumulation of toxic heavy metals such as copper. There are two experimental design schemes, one is to measure the algae biofilm by using the vacuum sample laser chamber, and the other is to use the special liquid sample laser chamber to analyze the microalgae target element in the liquid (see the figure below).


In recent years, researchers at CEITEC Laser Spectroscopy have conducted a series of studies on liquid elemental analysis, and are at the forefront of the world in the field of elemental analysis of LIBS liquids (including aqueous suspensions), providing a variety of optimal solutions. The following figure shows the scheme for analyzing heavy metal elements (copper) in water:

4, nano materials

Nanotechnology is a hotspot in today's materials technology, where Quantum dots (QDs) and their applications (such as quantum dot markers for life sciences, cancer cell imaging, etc.) are considered to be the most promising nanoparticles. In these applications, it is necessary to accurately detect QDs and qualitatively and quantitatively measure their spatial distribution. The current detection method is complex, time-consuming and expensive. The CEINEC/Atomtrace laser spectroscopy laboratory uses LIBS technology to treat different doses and different pH cadmium (Cd). The QDs solution was injected onto the filter paper, and the spatial distribution of QDs was measured and analyzed, which provided an important technical application scheme for the rapid detection of QDs by using LIBS technology.

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