Release date: 2014-06-23
Recently, in a research report published in the international magazine Analytical Chemistry, scientists from Rice University in the United States have developed a simple and high-accuracy detection technology to detect the signs of HIV and the degree of disease development in patients' bodies.
Researcher Rebecca Richards-Kortum said that the current gold standard for diagnosing HIV status in infants relies on laboratory equipment and currently available detection techniques to monitor viral load in their bodies, and researchers in this paper have developed A new nucleic acid-based HIV detection technology.
The new detection technology is based on a PCR-based method for amplification of recombinant polymerases (RPA), which replaces complex laboratory testing steps that allow rapid multi-amplification of genetic markers in the blood, resulting in genetic markers of the virus. The object can be easily detected; qRPA detection can be used to perform targeted labeling of fluorescent probes on specific sequences in HIV DNA, thereby using a machine for quantitative detection, and finally by software analysis of fluorescent DNA, doctors can master Dynamic information about HIV in the patient's body.
Professor Rohrman said that we can calibrate the test by amplifying the internal positive control and can use the same primers of the HIV sequence for amplification, so the test can be performed normally; this new technology can help track the viral load in the body of elderly patients. This provides some reference data for clinicians to monitor the HIV status of patients in real time to determine whether patients need timely treatment.
From a clinical perspective, DNA-based HIV testing can quantify the viral load of HIV in four orders of magnitude. Although current researchers use thermal cyclers for testing, researchers are working to develop a new type of test. The technology keeps the entire operating step between room temperature and body temperature, ensuring versatility and accuracy of the experiment.
Source: Laboratory Medicine Network
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