Release date: 2015-10-13
Researchers at Ben-Gurion University in Israel and the Massachusetts Institute of Technology in the United States have developed a highly sensitive, cost-effective technology that can quickly complete bacteria in air, soil, water and agricultural products in less than 24 hours. Detection of sexual pathogens.
According to the leader of the study, Dr. Ezra Olovsky, who is pursuing a doctorate at the Zuckerberg Water Institute at Ben-Gurion University, said, “The rapid and reliable detection of pathogens in the field samples is for public health and safety. And environmental monitoring is critical. The current methods used in food, water or clinical applications need to rely on the laboratory and spend a lot of time cultivating the technology, such as in the dairy industry, wastewater and runoff processes, in the environment The pathogen is monitored in real time in the sample.
The study, published online in the journal Water, Air & Soil Pollution, defines an accurate, inexpensive, high-throughput, and rapid alternative to detecting pathogens in different environmental samples. “This is the first comprehensive study of pathogen concentrations in a wide variety of environmental sample types, using standard (or traditional) methods to achieve multipathogen detection using complete parallel testing.
“We were able to accurately determine Salmonella in soil samples within 24 hours, whereas traditional methods took four to five days and needed to be classified,†said Dr. Ezraovsky. “We have also successfully identified a deadly infection, Pseudomonas aeruginosa, which is present in aerosols and is produced in domestic wastewater treatment systems. The results show that the technology we developed provides a wide range of methods. Relatively low concentrations of pathogenic microorganisms are detected quickly, efficiently, and reliably in challenging environmental samples.
To evaluate this technology, various types of environmental samples, including aerosols, various soil types, wastewater and vegetable surfaces (tomato), were simultaneously invaded by Salmonella or Pseudomonas aeruginosa. Researchers have chosen these pathogens because they are the main pathogen, have a high potential for survival in the environment, and are difficult to detect accurately at low concentrations.
“When this method was applied to on-site samples that did not invade the pathogen, our method was significantly better than the standard method and was able to detect the pathogen within one day of receipt of the sample,†Dr. Ezraovsky said. “Because this centralized and economical test procedure tells us where to focus on testing in a day, we don't need to spend a few days testing hundreds of samples and subsamples.â€
This rapid detection method uses two techniques at the same time, one is the evolutionary "MPN-type enrichment" technique for microbial detection, plus the "qPCR" technology widely used in molecular biology for real-time monitoring of DNA amplification.
“We have greatly shortened the previous solution and no longer used any expensive brand-name reagents to purify and purify the DNA, nor to increase the workflow, from the original sample to the qPCR experiment,†Ezra Olofs Dr. Ji said.
The detection of soil, water and vegetable samples is highly sensitive (down to every cell test) and the researchers believe that additional steps are needed to further increase the level of detection, allowing the technology to reflect low pathogen concentrations in aerosols (especially It is a low concentration of infectious agent).
Researchers recommend using this method to detect other pathogens such as Legionella, Staphylococcus aureus and Campylobacter jejuni, the second of which is the most common cause of foodborne illness.
Source: China Science and Technology Network
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