Model NO.: D-Bil
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*15 mL
Origin: Jinan, Shandong
Model NO.: D-Bil
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*15 mL
Origin: Jinan, Shandong
The direct bilirubin (D-Bil) assay kit provides a convenient and accurate measurement of concentration of D-Bil, also called conjugated bilirubin, in human plasma.
Test principle
In the presence of surfactant and indirect bilirubin inhibitors, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.
Product Features
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1. Linear range of D-Bil level: [0, 342] μmol/L
Linear correlation coefficient ∣r∣≥0.990Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.10 Â
3. Sensitivity
The change in absorbance (ΔmA) ≥ 0.30 while testing the specific D-Bil sample of concentration of 47 μmol/L.4. Accuracy
Relative deviation is within ±10%.5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤5.0%.Technical Parameters
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| Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
| Main wavelength | 450 nm | Secondary wavelength | 570 nm (Optional) |
| Method |
End-point method (dual reagents) |
Reaction direction | Negative |
| Sample | 8 μL | R1 | 240 μL |
| R2 | 60 μL | Reaction time | 300 s |
Packing Specifications
R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
Â
The direct bilirubin (D-Bil) assay kit provides a convenient and accurate measurement of concentration of D-Bil, also called conjugated bilirubin, in human plasma.
Test principle
In the presence of surfactant and indirect bilirubin inhibitors, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.
Product Features
Â
1. Linear range of D-Bil level: [0, 342] μmol/L
Linear correlation coefficient ∣r∣≥0.990Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.10 Â
3. Sensitivity
The change in absorbance (ΔmA) ≥ 0.30 while testing the specific D-Bil sample of concentration of 47 μmol/L.4. Accuracy
Relative deviation is within ±10%.5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤5.0%.Technical Parameters
Â
| Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
| Main wavelength | 450 nm | Secondary wavelength | 570 nm (Optional) |
| Method |
End-point method (dual reagents) |
Reaction direction | Negative |
| Sample | 8 μL | R1 | 240 μL |
| R2 | 60 μL | Reaction time | 300 s |
Packing Specifications
R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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Cardiac And Cerebral Vascular ,Cerebral Artery Occlusion ,Brain Infarction ,Anterior Cerebral Artery
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