Each bottle has an argon pressure pipe and a transfer pipe into the cap plug. For phosphorous amide, the pressure pipe of the No. 1-8 bottle also serves as the exhaust pipe. The argon tube is kept above the liquid level and the tube extends to the bottom of the bottle. When the valve is properly set to open, the upper space of the reservoir is pressurized by argon and the liquid is pushed into the delivery tube to its destination.
( 2 ) Pressure system
System pressure is provided by ultrapure argon ( 99.998 % ) . High argon density and low oxygen pollution make high pressure argon often used to activate vacuum assist systems and supply synthesizer regulators. Argon entering the synthesizer is passed through a 10 txm particle filter and sent to three pressure regulators that deliver argon to a specific pressure valve to increase the pressure of the reagent and solvent bottles. The regulator also supplies argon to the column and reagent valve body.
( 3 ) Reagents and solvent storage bottles
Each liquid storage bottle on the instrument has a specific position. The liquid storage cap holder corresponds to all the numbers on the instrument. The position on the instrument or the number of the liquid storage bottle is 1-8 ( the instrument with 5 bases is 1) ~ 5) , 9 , 10 , 11 , 12 , 14 , 15 , 18, and 19 ( equipment with automatic analysis ) . Push the No. 1 to No. 8 bottles up. There is an “O†ring around the Teflon plug, and a gas tight plug is formed inside each bottle neck. Because the reagent delivery system is pressurized, the sealing plug between the bottle and the instrument is kept airtight. Selecting a suitable disposable plug and adding an "O" ring to the bottle enhances air tightness.
( four ) phosphoramidite bottle exhaust
In addition to pressurization, the phosphoramidite bottles are vented with a pressurized exhaust pipe. Before putting the phosphoramidite on the instrument, use argon to remove the air and purify it. Purification is the delivery of gas through a delivery tube, and when the gas is delivered into the bottle, the air is expelled through the pressurized exhaust tube. This is done automatically by the bottle replacement step. The waste bottle is the low pressure side of the conveyor system and must be connected to the atmosphere. Make sure the exhaust pipe leads to the fume hood. If the exhaust pipe is blocked, back pressure will be generated and the transport of reagents and solvents will be suppressed.
( 5 ) Delivery valve block
The reagent delivery system consists of two reagent valve blocks ( 3 for 8 base instruments ) and 2 or 4 column valve blocks. The valve block controls the flow of gas and chemical reagents to the column and outlet. In addition to phosphoramidite and tetrazole, both reagents and solvents are delivered to all activation columns simultaneously. When there are multiple activation columns, subtle changes in flow rate are compensated by automatically adjusting the number of delivery times per column. Each 5- port column valve block directs the effluent from the waste port, the DMT collection port, or the DNA collection bottle, which also controls the argon gas used to remove or flush the reagents in the column and in the column valve block.
( 6 ) Road throttle valve
The reagent flows through the luer fitting at the bottom to the column. If one of the luer fittings below is not screwed, it should be found on a cylindrical glass flow path throttle. The reagent flows to the column through a small passage in the flow path throttle. A small amount of reagent can crystallize in the flow path throttle valve, which will cause the flow path to block for a long time.
( 7 ) Auxiliary vacuum
For proper operation of the valve block, the key is that the diaphragm forms a small gap in the dome. Each time the solenoid valve is opened, the air pump provides an auxiliary vacuum for each valve block. The auxiliary vacuum is formed on the solenoid side of the diaphragm to make the diaphragm A dome gap is formed.
( 8 ) Pillars
The nucleotides initially bound to the carrier are contained in a disposable column. In addition to the outside of the column, there are two fixed filter plates and two joints, all of which are made of an inert material. The fixed filter plate is a multi-ply L- type polystyrene fixed in the lids at both ends. Both the population and the exit are the parenter 's 1uer joints, paired with the instrument's road erection joint. The columns are symmetrical ( no top and bottom, front and back ) and can be connected to the highway erection in any way. Each column is numbered in color to indicate a different starting nucleotide and has a unique consecutive sequence number. The normal flow path is entered from the bottom, and the CPG particles are raised and kept in suspension by transporting the liquid upward. The flow rates of the solvent and reagents have been set to allow the particles to mix properly.
( 9 ) Waste liquid and exhaust
The final point of most chemical delivery is the waste bottle, which is an empty 10L polystyrene container that can be placed on the floor near the synthesizer or placed on a table below the instrument. The exhaust pipe directs the exhaust gas to a suitable exhaust device, such as a fume hood.
( 10 ) Conductivity pool
Conductive flow cells are designed to determine the total conductance of DMT cations released during each DNA synthesis cycle. The flow cell consists of two electrodes separated by a gap, with a small voltage applied between the gaps. The DMT cation acts as a conductance when flowing through the conductivity cell.
( 11 ) Battery
DNA synthesizers typically come with a lithium battery that can be used for several years. When the main power is disconnected, all synthesis parameters are retained. These parameters include stored DNA sequences, user-defined cycles, steps and functions, and bottle usage data. If a power failure is occurring in the synthesis, the synthesis will be interrupted and the synthesis can be restarted only when the main power is restored. The battery also keeps the clock inside the synthesizer.
( 12 ) Controller
The controller directs and initiates all activities of the synthesizer, the main part of which is software, microprocessors, display screens, keypads and related electronic components. The software controls all necessary operations of the synthesis and is interpreted and executed by the microprocessor. The software is stored in a removable memory card that is inserted in the back of the instrument. The composite information is displayed on the LCD screen and can be communicated to the instrument by selecting the keys on the keypad. The software is " menu-driven " and can be commanded by pressing an appropriate button to select an item. To complete the automated synthesis, the software applies a cycle of a series of steps to complete the entire chemical reaction .
The Network Camera/ Network IR Camera/ Network Cameras/ Network Camera HD/ IP Camera Wifi/ IP Cameras/ Mini IP Cam, also called the IPC, WEBCAM, or WEB Camera, is a new-generation camera that combines the traditional camera and the network technology. And the user can monitor the Network Camera images just with a standard web browser (such as the "Microsoft IE or Netscape).
As a new generation product that combines the traditional camera and the network video technology, in addition to the image capturing function of the traditional cameras, the Network Camera also has a built-in digital compression controller and a WEB-based operating system; which makes the video data compressed and encrypted, and then delivered to the end users via the local area network, internet or wireless network. The remote user can use a standard web browser on the PC to access the network camera according to the IP address of the network camera, monitor the on-site situation of the target site, edit and store the image data, and can even control the camera PTZ and lens to achieve the monitor in all directions.
Nowadays, the Network Camera are being used more and more widely. So do you know how the Network Camera was invented? Well, here is a story.
The design of the world's first Network Camera is originated from two scientists who wanted to drink coffee.
In 1991, only the main computer room of the Cambridge University Computer Research Center had a coffee maker. From time to time, scientists from other rooms ran to the main computer room but found that the coffee was drank up. In order to solve the problem of a wasteful trip when pouring coffee, scientists Fraser and Paul thought of assembling a device that can monitor the coffee maker in the main room. They first pointed a camera at the coffee maker, set to take three photos per minute, and then wrote a program to send the camera pictures to the research department`s internal network. Then, the Cambridge University successfully installed the first Network Camera in the world.
On November 22nd, 1993, the real "Internet camera" was born and it was still in the computer research department of the Cambridge University. The laboratory which another scientist named Johnson was at could not be connected to the internal network. Johnson could not use the previous monitoring software to check the coffee, so he wrote a program to make his own computer can receive photos from the camera; which makes the breakthrough of the camera from the internal network to the World Wide Web, realized.
Since then, millions of people from all over the world have joined the "coffee pot watching activity" through the Internet. In 2011, due to the obsolete equipment and the inability to maintain it, Cambridge computer scientists finally shut down the Network Camera.
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