The importance of temperature to CCD from in vivo imaging technology

The emergence of in vivo imaging technology has made the study of molecular and cell biology no longer just in vitro. The researchers can clearly observe the gene expression and cellular activities in vivo through in vivo imaging technology. This technology is widely used in medical and biological research. This live imaging technique was first discovered by scientists at Stanford University in the United States. They used the thinning of the back and the back-illuminated cold CCD in a closed-closed black box to make the monitoring of living animals become a reality.

In vivo imaging technology is realized by the thinning of the back and the generation of cold CCD by back radiation. Therefore, we can say that without the thinning of the back and the back-illuminated cold CCD, there will be no birth and development of living body imaging technology. In order to ensure the effective implementation of live imaging technology, the sensitivity and signal-to-noise ratio of CCD are constantly increasing. The lower the CCD temperature, the smaller the dark current and electronic noise, and the higher the signal-to-noise ratio. The sensitivity is correspondingly improved. Therefore, the temperature of the CCD can be said to be the core influencing factor of the living imaging technology.

The back thinning and back-illuminated cold CCD is precisely because it has an ultra-low temperature CCD chip, which greatly enhances the signal-to-noise ratio, which is enough to affect the generation and development of living imaging technology, and the CCD is also weak for animals. With the extremely high sensitivity and the double positive factors, this technology has achieved the best results. It has been widely favored and applied for a while, and has achieved great results in the fields of oncology and gene therapy. Achievements have greatly promoted the development of biomedical imaging in molecular medicine.

In summary, we have seen the importance of temperature to CCD through in vivo imaging technology. The signal-to-noise ratio of the CCD increases as the temperature decreases. From the perspective of thermodynamics, the higher the temperature, the greater the kinetic energy generated by electrons, and the more inactive signals in the process of photoelectric conversion and transfer, the more serious the influence of noise in the CCD imaging process, and the signal-to-noise ratio will be drastically reduced. . Conversely, if the temperature is lowered, the signal-to-noise ratio will increase; the temperature drop will prevent CCD aging. It is well known that high temperatures accelerate the aging of electronic devices, which is why many manufacturers install heat sinks for CCDs in order to reduce the effect of temperature on the CCD signal-to-noise ratio; high temperatures can form dark currents. The higher the temperature, the greater the possibility of dark current generation, and the formation of dark current affects the sensitivity of the CCD, which is not conducive to the generation of high quality images.