CultreCoat® Cell Adhesion Assay Kit Principles and Instructions for Use

Cell adhesion is the related role between cells and extracellular machinery molecules. It plays an important role in tumor cell metastasis, infiltration, embryogenesis, etc. At the same time, many cells are needed in the process of normal cell growth and tissue differentiation. The adhesion function works. In the process of tissue gap transfer of tumor cells, the extracellular matrix is ​​a necessary part of it. Extracellular matrix ECM is an intricate network of macromolecules. Tumor cells need to interact with ECM before and during metastasis, and the cytoskeleton associated with cell mobility forms a complex, which ultimately performs metastasis. The extracellular matrix is ​​mainly composed of collagen (collagen) and non-collagen glycoprotein (such as fibronectin, laminin and the like).

Conventional cell adhesion assays are currently performed by including extracellular matrix proteins (or individual components) embedded in cell culture plates. However, these traditional methods of cell behavior analysis have been criticized by researchers because of the following defects: 1. Higher experience on the operator, such as uniform deposition of matrix proteins and optimization of reagents and processes, it is difficult to achieve higher The stability of the experimental batch; 2, the cell matrix used for purchase did not undergo strict quality verification, and had great influence on cell behavior and even activity, and the final data lacked credibility; 3. Manual cell counts existed in certain random experiments. Sex. Here, Agilent Technologies recommends Trevigen's CultreCoat® and Cultre® full range of tumor cell behavioral assays to provide cell invasion, cell migration, cell adhesion, in vitro vascular regeneration, and vascular permeability analysis. And other complete tumor cell behavior analysis products and kits.

The cell adhesion analysis solution provided by Trevigen has the following characteristics:

  1. Researchers can directly purchase pre-embedded kits of matrix components of different components of CultreCoat according to their own application and needs, or they can only purchase the Cultrex basement membrane extract components after strict biological function test;
  2. Since different cell lines have multiple adhesion potentials for different matrix components, Trevigen also offers the CultreCoat Matrix Protein Array Cell Adhesion Assay Kit designed to select the most appropriate BME component;
  3. Trevigen's cell adhesion assay provides fully quantified cell adhesion analysis without the need for manual cell counting; Trevigen's patented BME matrix and other component proteins are pre-embedded on microplates with very low cytotoxicity. It is verified by strict bacteria-free/fungal/mycoplasma, which is close to the real environment. At the same time, the fluorescence quantification scheme can guarantee higher precision.

The CultreCoat® Cell Adhesion Assay Kit provides a protocol for assessing the effects of cell-matrix interactions. The assay is simple and uses a standardized 96-well high-throughput assay. The black strip holes reduce the background and provide greater sensitivity and flexibility to the number of samples evaluated. The Calcein-AM label provides a direct comparison of cell load and adherent cell volume, based on the final fluorescence estimate to provide a load reference and percentage of adherent cells per well. Standard references are provided to determine background and non-specific binding.

Product name Brand Item number product manual
CultreCoat® BME 96 Well Cell Adhesion Assay Trevigen 3490-096-K 96 fluorescence analysis
CultreCoat® Laminin I 96 Well Cell Adhesion Assay Trevigen 3491-096-K 96 fluorescence analysis
CultreCoat® Collagen I 96 Well Cell Adhesion Assay Trevigen 3492-096-K 96 fluorescence analysis
CultreCoat® Collagen IV 96 Well Cell Adhesion Assay Trevigen 3493-096-K 96 fluorescence analysis
CultreCoat® Fibronectin 96 Well Cell Adhesion Assay Trevigen 3494-096-K 96 fluorescence analysis
CultreCoat® Vitronectin 96 Well Cell Adhesion Assay Trevigen 3495-096-K 96 fluorescence analysis


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Store below 30c in a dry place away from heat and direct sunlight.







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